caspase-3、Ki-67在膀胱移行细胞癌中的表达及临床意义
Expression and significance of caspase-3 and Ki-67 in bladder transitional cell carcinomas
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摘要: 目的:探讨凋亡同胱氨酸蛋白-3(caspase-3)、增殖细胞核抗原Ki-67在膀胱移行细胞癌(bladder transitional cell carcinomas,BTCC)的表达及其与肿瘤分级、分期之间的关系。方法:采用免疫组织化学SP法,对55例BTCC和10例正常膀胱黏膜caspase-3和Ki-67的表达进行检测,分析两者在膀胱癌组织和非膀胱癌组织中的表达及表达水平与BTCC病理学分级、临床分期的关系。结果:caspase-3在正常膀胱黏膜中的阳性表达率为90.0%,膀胱癌组织中的阳性表达率为47.3%,两者阳性表达率差异有统计学意义(P<0.05),同时发现caspase-3的阳性表达率随膀胱癌的组织分化程度的降低而表现出下降的趋势,差异有统计学意义(P<0.05)。Ki-67在正常膀胱黏膜中没有发现阳性表达病例,在膀胱癌中的阳性表达率为52.7%,差异有统计学意义(P<0.01),Ki-67的阳性表达率随肿瘤组织分化程度的降低和临床分期的增高表现出上升趋势,且差异有统计学意义(P<0.05)。结论:caspase-3参与了BTCC的发生过程,可以作为评价BTCC侵袭性的一个指标。Ki-67的阳性表达率增高与BTCC的发生、发展可能有一定关系。Abstract: Objective: To investigate the expression of caspase-3 protein and Ki-67 in bladder transitional cell carcinomas (BTCC) and its relationship with the grading and staging of the tumor.Methods: The expression of caspase-3 protein and Ki-67 in 55 cases of bladder transitional cell carcinomas and 10 cases of normal bladder mucosas were determined by immuohistochemisitry (SP method).Results: The positive expression of caspase-3 in BTCC (47.3%) was significantly lower than that in normal bladder mucosas (90.0%).The expression level of caspase-3 was positively correlated with the histological grades of BTCC (P<0.05).No positive expression of Ki-67 was observed in normal bladder mucosas,while it was 52.7% in BTCC (P<0.01).The expression of Ki-67 was related positively with the histological grade and negatively with the tumor stage of BTCC (P<0.05).Conclusions: Caspase-3 is closely correlated with the tumorigenesis of BTCC,and may act as a useful marker of invasion of BTCC.Increased expression of Ki-67 maybe related with the genesis and development of BTCC.
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