赵素容, 霍强, 李见春, 蒋志文. 高效液相色谱法测定人血清中氨苄西林、丙磺舒的浓度研究[J]. 蚌埠医学院学报, 2011, 36(5): 516-518,522.
    引用本文: 赵素容, 霍强, 李见春, 蒋志文. 高效液相色谱法测定人血清中氨苄西林、丙磺舒的浓度研究[J]. 蚌埠医学院学报, 2011, 36(5): 516-518,522.
    ZHAO Su-rong, HUO Qiang, LI Jian-chun, JIANG Zhi-wen. Determination of ampicillin and probenecid in human serum by high performance liquid chromatography[J]. Journal of Bengbu Medical College, 2011, 36(5): 516-518,522.
    Citation: ZHAO Su-rong, HUO Qiang, LI Jian-chun, JIANG Zhi-wen. Determination of ampicillin and probenecid in human serum by high performance liquid chromatography[J]. Journal of Bengbu Medical College, 2011, 36(5): 516-518,522.

    高效液相色谱法测定人血清中氨苄西林、丙磺舒的浓度研究

    Determination of ampicillin and probenecid in human serum by high performance liquid chromatography

    • 摘要: 目的:建立测定人血清中氨苄西林、丙磺舒浓度的高效液相色谱法。方法:(1)氨苄西林,以诺氟沙星为内标,血清样品用0.6%的高氯酸沉淀蛋白后进行测定;色谱柱为HigginsTMC18柱(250 mm×4.6 mm,5μm);流动相为有机相(甲醇-乙腈-异丙醇,100∶2∶5,v/v):水相(0.01 mol/L磷酸二氢钾,pH3.5)=23∶77;检测波长210 nm;流速1.0 ml/min;柱温30℃。(2)丙磺舒,以缬沙坦为内标,血清样品用乙腈沉淀蛋白后进行测定;色谱柱为HigginsTMC18柱(250 mm×4.6 mm,5μm);流动相为有机相(甲醇-乙腈,400∶275,v/v):水相(乙酸-水,1∶60,v/v)=77∶23;检测波长280 nm;流速1.0 ml/min;柱温30℃。结果:氨苄西林在0.14~11.2μg/ml浓度范围内线性关系良好(r=0.999 5),最低检测限为0.14μg/ml;丙磺舒在0.20~16.0μg/ml浓度范围内线性关系良好(r=0.999 1),最低检测限为0.20μg/ml。结论:高效液相色谱法操作简便、灵敏、专一性好,适用于人体药动学研究。

       

      Abstract: Objective: To establish a method to determine the concentration of ampicillin and probenecid in human serum by high performance liquid chromatography(HPLC).Methods: (1)Ampicillin was detected by HPLC using norfloxacin as internal standard after sample was sedimented with 0.6% perchloric acid.HigginsTM C18 column(250 mm×4.6 mm,5 μm) was used,the mobile phase consisted of organic phase(methanol-acetonitrile-isopropanol,100:2:5,v/v) and aqueous phase(0.01 mol/L potassium dihydrogen phosphate,pH 3.5) with a ratio of 23:77,the flow rate was 1.0 ml/min,the detection wavelength was set at 210 nm,and column oven at 30℃.(2)Probenecid was detected by HPLC using valsartan as internal standard after sample was sedimented with acetonitrile.HigginsTM C18 column(250 mm×4.6 mm,5 μm) was used,the mobile phase consisted of organic phase(methanol-acetonitrile,400:275,v/v) and aqueous phase(acetic acid-water,1:60,v/v) with a ratio of 77:23,the flow rate was 1.0 ml/min,the detection wavelength was set at 280 nm,and column oven at 30℃.Results: Good linearity was obtained within the range of 0.14-11.2 μg/ml for ampicillin(r=0.999 5),and 0.20-16.0 μg/ml for probenecid(r=0.999 1).The mininum detectable concentrations for ampicillin,and probenecid were 0.14 μg/ml and 0.20 μg/ml,respectively.Conclusions: This method is simple,sensitive and specific,which can be applied to study the pharmacokinetics of ampicillin and probenecid.

       

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