何金根, 潘家华, 楼皖玲, 刘辉. 维生素A和布地奈德对哮喘大鼠外周血T细胞亚群和调节性T细胞的影响[J]. 蚌埠医学院学报, 2011, 36(5): 452-455.
    引用本文: 何金根, 潘家华, 楼皖玲, 刘辉. 维生素A和布地奈德对哮喘大鼠外周血T细胞亚群和调节性T细胞的影响[J]. 蚌埠医学院学报, 2011, 36(5): 452-455.
    HE Jin-gen, PAN Jia-hua, LOU Wan-ling, LIU Hui. The effects of vitamin A and budesonide on serum T-lymphocyte subsets and CD4+CD25+ regulatory T cells in rat asthma model[J]. Journal of Bengbu Medical College, 2011, 36(5): 452-455.
    Citation: HE Jin-gen, PAN Jia-hua, LOU Wan-ling, LIU Hui. The effects of vitamin A and budesonide on serum T-lymphocyte subsets and CD4+CD25+ regulatory T cells in rat asthma model[J]. Journal of Bengbu Medical College, 2011, 36(5): 452-455.

    维生素A和布地奈德对哮喘大鼠外周血T细胞亚群和调节性T细胞的影响

    The effects of vitamin A and budesonide on serum T-lymphocyte subsets and CD4+CD25+ regulatory T cells in rat asthma model

    • 摘要: 目的:探讨维生素A(Vit A)和布地奈德对哮喘大鼠外周血T细胞亚群和CD4+CD25+调节性T细胞(CD4+CD25+ regulatory Tcell,CD4+CD25+ Treg)的影响,为其在哮喘防治中的应用提供实验依据。方法:将42只SD大鼠用卵蛋白建立哮喘模型,随机分成布地奈德治疗组、Vit A治疗组和哮喘对照组,每组各14只,各组干预1周,于第4周和第5周取外周血50μl,流式细胞术检测T细胞亚群及Treg细胞变化。结果:激发后第4周,Vit A组CD3+T细胞、CD4+CD25+ Treg细胞显著高于布地奈德组(P<0.05),CD3+CD8+T细胞均低于布地奈德组(P<0.05);布地奈德组CD4+CD25+ Treg细胞显著低于哮喘组(P<0.01);激发后第5周,布地奈德组CD4+CD25+ Treg细胞低于哮喘组(P<0.05)。结论:雾化吸入Vit A可减轻哮喘大鼠炎症反应,这一作用可能与哮喘大鼠外周血CD3+T细胞和CD4+CD25+ Treg细胞升高、CD8+T细胞降低有关。

       

      Abstract: Objective: To investigate the effects of vitamin A and budesonide on peripheral blood T-lymphocyte subsets and CD4+CD25+ Treg cells in rat asthma model,which provide the experimental basis for vitamin A in the prevention and treatment of bronchial asthma.Methods: Forty-two adult female SD rats were divided into three groups,the budesonide group,vitamin A group and asthma group.There were 14 rats in each group.The peripheral blood was conducted to determinate T-lymphocyte subsets and CD4+CD25+ Treg by flow cytometry.Results: After the 4th of stimulation,CD3+T cell and CD4+CD25+ Treg cells in vitamin A group were significantly higher than those in budesonide group.CD3+CD8+T cell in vitamin A group was significantly lower than that in asthma group(P<0.05).CD4+CD25+ Treg cells of budesonide group was significantly lower than that in asthma group(P<0.01).After the 5th of stimulation,CD4+CD25+ Treg cells in budesonide group was significantly lower than that in asthma group(P<0.05).Conclusions: Inhalation of vitamin A can reduce inflammation in asthmatic rats,which may be associated with the increases of peripheral blood CD3+T cells and CD4+CD25+ Treg cells cells and the decrease of CD8+T cells.

       

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