Abstract: Objective: To observe the clinical characteristics of chronic hepatitis B patients with hepatitis E overlap-infection,and the influence of hepatitis E virus on patients with chronic hepatitis B.Methods: The clinical features of 40 patients with chronic hepatitis B overlapped with hepatitis E super infection and 40 patients with pure chronic hepatitis B infection were analyzed and compared.Results: The total bilirubin,alanin amino transferase,the aspartic acid amino transferase,the zymoplasm original activity and the blood albumin of the two groups differed significantly (P<0.05-P<0.01);the negative rate of HBeAg and the positive rate of HBV-DNA of the super infection group were lower than those of the control group (P<0.05).Conclusions: The chronic hepatitis B patients with hepatitis E overlap infection may resulted in acute aggravation and critical illness,but hepatitis E virus has certain inhibitory effect on the duplication of hepatitis B virus.