Abstract: Objective: To study the distribution and drug susceptibility of pathogenic bacteria in the bile samples.Methods: The distribution and drug resistance of 634 strains of pathogenic bacteria isolated from the bile samples were analyzed retrospectively.Results: Among the 634 strains isolated,248 were Gram positive bacteria,369 Gram negative bacteria and 27 fungus.Two hundred and ten strains of the G⁺ bacteria were Enterococcus and 162 strains of G^- bacteria were E.coli.G⁺ bacteria were the most susceptible to vancomycin,while G^- bacteria were the most sensitive to amikacin.Conclusions: As the amount of pathogenic bacteria,the distribution of microbial population in the bile and their drug resistance to antibiotics are changing,proper antibiotics should be chosen in accordance to the results of germiculture.