Abstract: Objective: To investigate the expression and association of macrophage migration inhibitory factors(MIF) and vascular endothelial growth factor-C(VEGF-C) in the breast cancer and the adjacent normal tissues. Methods: Immunohistochemistry Elivision was used to detect the expressions of MIF and VEGF-C protein in 75 breast cancer and the adjacent normal breast tissues. The relationship between their expression and the clinical pathology was analyzed. Results: The positive expression rates of MIF and VEGFC in the tissue of breast cancer were 85.33% and 69.33% respectively,which was obviously higher than that in the corresponding adjacent normal breast tissues(P < 0.05); the positive expression rates of MIF and VEGF-C in the lymph node positive group were 95.24% and 83.33% respectively,which was obviously higher than that in the lymph node negative group(P < 0.01); the expression of MIF and VEGF-C had positive correlation in the tissues of breast cancer(P < 0.05); the expression of MIF were positively correlated with the tumor size(P < 0.01),and the expression of VEGF-C had positive correlation with the histological grade(P < 0.05). The positive expression of MIF and VEGF-C had no significant difference in the age, clinical stage, ER, PR or cerbB-2 (P > 0.05). Conclusions: It suggests that high expression of VEGF-C and MIF may promote the metastasis of breast carcinoma by mutual adjustment through some mechanism.