Abstract: Objective: To explore the problems existing in human immunodeficiency virus(HIV) antibody confirmatory testing techniques and the corresponding solutions in practical work by following up one case of false positive conclusion in HIV antibody confirmatory test.Methods: The subject underwent HIV antibody screening and confirmatory testing,and received follow-up testing at the 4th week,8th week and 12th week.In the last two follow-ups,HIV-1 viral load testing was conducted;and in the fourth follow-up,the CD4⁺ T lymphocytes were detected.Results: The band types of HIV antibody detected by immunoblotting in the four tests were all gp160,gp120 and p24;the viral load results in the last two follow-ups were both TND,and the result of CD4⁺ T lymphocytes in the fourth test was 885 cells/μl.Combined with epidemiological data,the HIV antibody was ultimately determined to be negative.Conclusions: With the increasing of inconclusive samples by HIV antibody confirmatory test,we must be greatly cautious of the confirmatory positive samples in the borderline state.For certain special samples,testers must combine epidemiological data analysis and follow-up testing to make an accurate test report.