朱磊, 傅鸣郁, 王明光, 田静, 何秀华, 安新江. 银杏叶提取物对急性心肌梗死大鼠心肌细胞的保护作用[J]. 蚌埠医学院学报, 2011, 36(4): 335-338.
    引用本文: 朱磊, 傅鸣郁, 王明光, 田静, 何秀华, 安新江. 银杏叶提取物对急性心肌梗死大鼠心肌细胞的保护作用[J]. 蚌埠医学院学报, 2011, 36(4): 335-338.
    ZHU Lei, FU Ming-Yu, WANG Ming-guang, TIAN Jing, HE Xiu-hua, AN Xing-jiang. Protection of Ginkgo biloba extract on cardiomyocyte after acute myocardial infarction in rats[J]. Journal of Bengbu Medical College, 2011, 36(4): 335-338.
    Citation: ZHU Lei, FU Ming-Yu, WANG Ming-guang, TIAN Jing, HE Xiu-hua, AN Xing-jiang. Protection of Ginkgo biloba extract on cardiomyocyte after acute myocardial infarction in rats[J]. Journal of Bengbu Medical College, 2011, 36(4): 335-338.

    银杏叶提取物对急性心肌梗死大鼠心肌细胞的保护作用

    Protection of Ginkgo biloba extract on cardiomyocyte after acute myocardial infarction in rats

    • 摘要: 目的: 探讨银杏叶提取物(EGb761)对急性心肌梗死(AMI)大鼠心肌细胞的保护作用。方法: 雄性SD大鼠30只,随机均分为假手术组(A组)、AMI组(B组)和EGb761组(C组)。C组腹腔注射EGb761(20 mg/kg),A组和B组腹腔注射等容量的生理盐水,每天1次,共3天。实验大鼠于第3天开胸,A组冠脉下穿线但不结扎冠状动脉,B组和C组结扎冠状动脉左前降支,制备AMI模型。实验终点,取腹主动脉血测定血清中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,观察各组大鼠心肌细胞凋亡指数(AI)、细胞色素C (Cytc)和Caspase-9表达以及线粒体膜电位的变化。结果: 与B组相比,C组SOD活性明显增高,MDA含量显著下降,AI及Caspase-9表达明显降低,Cytc胞质表达显著减少而线粒体表达显著增加,线粒体膜电位明显升高(P<0.01)。结论: EGb761可减少AMI缺血区心肌细胞凋亡,其机制可能与减轻氧化应激,保护线粒体功能,减少Cyt-c释放入胞质有关。

       

      Abstract: Objective: To study the protective effect of Ginkgo biloba extract (EGb761) on cardiomyocyte after acute myocardial infarction (AMI) in rats.Methods: Thirty male Sprague-Dawley rats were randomly divided into sham group (group A),AMI group (group B),EGb761 group (group C),ten in each group.In group C,EGb 761 (20 mg/kg) was injected intraperitoneally,in group A and group B,normal saline (20 mg/kg) was injected intraperitoneally.All the liquid was injected once a day for 3 days.The animal model was established by ligating left anterior descending coronary artery on the third day.The operation in group A was similar to group B except for ligating the left anterior descending coronary artery.At the end of experiment,the levels of the superoxide dismutase (SOD),malondialdehyde (MDA),apoptotic index (AI),mitochondrial membrane potential and the expression of Cyt-c and caspase-9 were measured.Results: Compared with group B,the contents of MDA,AI,caspase-9 and cytoplasmic Cyt-c were significantly lower in group C (P<0.01).The level of SOD,the expression of mitochondrial Cyt-c and mitochondrial membrane potential were significantly higher in group C (P<0.01).Conclusions: EGb 761 can decrease apoptosis in ischemic myocardium after acute myocardial infarction.The mechanism is possibly that EGb 761 can decrease oxidative stress,protect mitochondrial functions and reduce the release of Cyt-c from mitochondria to cytoplasma.

       

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