抗DNP、Sm、ds-DNA抗体检测在系统性红斑狼疮诊断中的应用

张强; 李玉云; 王丽

抗DNP、Sm、ds-DNA抗体检测在系统性红斑狼疮诊断中的应用

The value of detecting anti-DNP antibody, anti-Smith antibody and anti-double stain DNA antibody in diagnosis of SLE

摘要

摘要: 目的: 探讨抗DNP抗体、抗Sm抗体、抗ds-DNA抗体联合检测在系统性红斑狼疮(SLE)诊断中的价值。方法: 用免疫印迹法检测抗Sm抗体,免疫斑点法检测抗ds-DNA抗体,胶乳免疫法检测抗DNP抗体。结果: 57例SLE患者,抗DNP抗体阳性率为73.7%,抗Sm抗体阳性率33.3%,抗ds-DNA抗体阳性率61.4%,三者联合检测阳性率达94.7%。结论: 抗DNP抗体、抗Sm抗体、抗ds-DNA抗体联合检测可提高对SLE诊断的阳性率;对SLE病情的追踪和预后判定有重要作用;用抗DNP抗体检测代替LEC检测可直接检测血清中的狼疮因子,弥补了因实验条件所致LEC检测阳性率低等缺陷。

Abstract: Objective: To investigate the significance of anti-DNP antibody,anti Smith antibody and anti-double stain DNA antibody in the patient with systemic lupus erythematosus(SLE).Methods: Anti-Smith antibody was detected by immuno-blotting technique,anti-double stain DNA antibody by dot immunofiltration assay,and anti-DNP antibody by latex immunoassay.Results: Among the 57 cases,the positive rates of anti-DNP antibody,anti-Smith antibody, and anti-double stain DNA antibody were 73.7%,33.3% and 61.4%,respectively.The total positive rate was 94.7%.Conclusions: In diagnosing SLE,allied examination of anti-DNP antibody,anti Smith antibody,anti-double stain DNA antibody can upraise the positive rate.The above tests are important in tracing patient's condition and judging the prognosis of SLE.Detecting serum anti-DNP can directly improve the detection rate of SLE instead of LE cell(LEC) detection.

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