The effects of endomorphin-1 postconditioning in rat with myocardial ischemia reperfusion injury
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摘要: 目的: 观察内吗啡肽-1(EM-1)后处理对在体大鼠心肌缺血再灌注损伤(IRI)的保护作用,并初步探讨EM-1对IRI可能的保护作用.方法: 雄性SD大鼠24只,随机分为4组:假手术组(S组)、缺血复灌组(I/R)、缺血后处理组(IPO组)和EM-1后处理组(EM组).S组于大鼠冠状动脉左前降支下穿线不结扎维持150 min;I/R组结扎大鼠冠状动脉左前降支30 min,再灌注120 min复制缺血再灌注模型,IPO组和EM组分别于再灌注前5 min,静脉给予0.9%氯化钠注射液0.5 ml和EM-1 20μg/kg,再灌注120 min.动态监测血流动力学指标的变化;再灌注结束后取动脉血浆检测丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性;光学显微镜检查心肌细胞形态.结果: 与S组比较,I/R组各时间点心率、平均动脉压和心率-血压乘积均显著降低(P<0.05~P<0.01);形态学显示心肌细胞损伤明显;血浆MDA含量增高,SOD活性下降(P<0.01).与I/R组比较,除EM组再灌注120 min 平均动脉压无明显增高外,IPO组和EM组各时间点心率、平均动脉压和心率-血压乘积均有所增高(P<0.05~P<0.01);形态学显示IPO组和EM组心肌细胞损伤减轻;IPO组和EM组血浆MDA含量均降低,SOD活性增加(P<0.01).结论: EM-1后处理对心肌IRI产生保护作用,其可能通过抗氧化应激损伤发挥心肌保护作用.Abstract: Objective: To investigate the effects of endomorphin-1(EM-1) postconditioning in rat with myocardial ischemia-reperfusion injury (IRI) in vivo and to assess its mechanism.Methods: twenty-four male Sprague Dawley rats were randomly divided into 4 groups:sham group(S group),ischemia-reperfusion group(I/R group), ischemia postconditioning group (IPO group),EM-1 postconditioning group(EM group).S group was established through threading under the left anterior descending branch of coronary artery and no occluding for 150 min;I/R group was established through occluding the left anterior descending branch of coronary artery for 30 min and reperfusion for 120 min;IPO group and EM group were respectively intravenous injection 0.9% NaCl 0.5ml and EM-1 20 μg/kg on 5 min before reperfusion,then reperfusion for 120 min.Dynamic index of blood flow was recorded and analyzed. At the end of reperfusion, arterial blood sample was obtained to measure plasma contents of malondialdehyde(MDA) and the activities of superoxide dismutase(SOD);the rats were sacrificed for assessment cell morphology of light microscopy. Results: Compared to S group,the heart rate,mean arterial pressure and rate-pressure product were decreased in I/R group(P<0.05~P<0.01);the contents of MDA was significantly increased and the activities of SOD was significantly decreased in I/R group(P<0.01);in cell morphology of light microscopy,the damage of myocardial structure was significantly increased in I/R group.The heart rate,mean arterial pressure and rate-pressure product in IPO and EM group were increased compared to I/R group except of MAP at 120 min following reperfusion in EM group(P<0.05~P<0.01),the contents of MDA in IPO and EM group was decreased and the activities of SOD was increased compared to I/R group(P<0.01),in cell morphology of light microscopy,the damage of myocardial structure was significantly reduced in IPO and EM group.Conclusions: EM-1 postconditioning can relieve ischemia reperfusion injury in myocardial protection,EM-1 postconditioning produces the protective effect by reducing the MDA and increasing the SOD.
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