Abstract: Objective: To explore the changes of liver and kidney function indicators in human immunodeficiency virus(HIV) infected blood donors co-infected with hepatitis C virus(HCV) or hepatitis B virus(HBV). Methods: The serological markers of HCV and HBV,and all indicators of liver and kidney were detected among 125 HIV infected blood donors and 40 health cases as control. Results: The infection rate of HCV was 92.0% and 5.6% of HBV among 125 HIV infected blood donors. There were statistical differences of ALT, AST between HIV co-infected with HCV donors and health control cases, also with HIV infected alone donors. In contrast to health control group,there was significant difference of β₂-MG in HIV co-infected with HBV or HCV and HIV infected alone donors. There was significant difference of β₂-MG between HIV co-infected with HCV and HIV infected alone donors(P < 0.01). Conclusions: HIV co-infected with HBV or HCV may accentuate the damage of liver function, and it seems no effect on kidney function. The rise of β₂-MG may be the over-activation of host immunity.