三氧化二砷对黑色素瘤B<sub>16</sub>细胞端粒酶活性影响

三氧化二砷对黑色素瘤B₁₆细胞端粒酶活性影响

摘要: 目的: 研究三氧化二砷(As₂O₃)对黑色素瘤B₁₆细胞端粒酶活性的作用,探讨砷及其化合物治疗黑色素瘤的作用机制,为其治疗黑色素瘤提供新的理论和实验依据。方法: 用TRAP-ELISA和TRAP-PAGE银染法检测黑色素瘤B₁₆细胞(简称B₁₆)端粒酶活性。结果: As₂O₃对黑色素瘤B₁₆细胞端粒酶活性的抑制有明显的浓度和时间依赖关系。结论: As₂O₃对黑色素瘤B₁₆细胞端粒酶活性表达的抑制作用随药物浓度的增加或作用时间的延长而增强。

Abstract: Objective: To study the influence of arsenic trioxide(As₂O₃) on the telomerase activity in melanoma B₁₆ cells,so as to provide new theoretical and experimental basis for the treatment of melonoma by As₂O₃.Methods: The telomerase activity in melonoma B₁₆ cells was detected by TRAP-ELISA and TRAP-PAGE-silver staining.Results: As₂O₃ could inhibit telomerase activity in melanoma B₁₆ cells and the rates of inhibition had both dose-dependent and time-dependent manner.Conclusions: The inhibition of B₁₆ cells by As₂O₃ is enhanced with the increase of As₂O₃ concentration or prolongation of co-culture time.