系统性红斑狼疮患者外周血CD4<sup>+</sup>CD25<sup>high</sup>Treg上PD-1分子的表达分析

王元元; 陈晓蓉; 刘铜; 唐洁; 谢长好

系统性红斑狼疮患者外周血CD4⁺CD25^highTreg上PD-1分子的表达分析

1. 安徽医科大学组织学与胚胎学教研室, 安徽合肥 230032;
2. 蚌埠医学院组织学与胚胎学教研室, 安徽蚌埠 233030;
3. 感染与免疫安徽省重点实验室, 安徽蚌埠 233030;
4. 蚌埠医学院第一附属医院风湿免疫科, 安徽蚌埠 233004

基金项目:

安徽省卫生厅青年基金资助项目(09B108)

详细信息

作者简介:
王元元(1979-),女,讲师.

中图分类号: R593.24
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出版历程
- 收稿日期: 2011-07-19

Analysis of frequency and expression of programmed cell death-1 of CD4⁺CD25^highTreg peripheral blood in systemic lupus erythematosus patients

1. Department of Histology and Embryology, Anhui Medical University, Hefei Anhui 230032;
2. Department of Histology and Embryology, Bengbu Medical College, Bengbu Anhui 233004;
3. Key Laboratory of Infection and Immunity in Anhui, Bengbu Medical College, Bengbu Anhui 233004;
4. Department of Rheumatology, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004, China

摘要

摘要: 目的:检测系统性红斑狼疮(systemic lupus erythematosus,SLE)患者外周血CD4⁺CD25^highTreg及其程序性死亡受体-1(programmed death-1,PD-1)分子的表达,探讨Treg和PD-1表达在SLE中的意义。方法:流式细胞仪检测SLE患者组、正常对照组外周血CD4⁺T细胞中的CD4⁺CD25^highTreg和CD4⁺CD25^lowT细胞的比例,及其PD-1分子的表达率。结果:CD4⁺CD25^highTreg在SLE患者组外周血中CD4⁺T细胞的比例(0.63±0.31)%与正常对照组(2.07±0.74)%相比明显降低(P<0.05),该亚群细胞上PD-1⁺的百分率(24.99±18.65)%与对照组(6.97±1.92)%相比显著增高(P<0.05);CD4⁺CD25^lowT细胞的百分率(13.40±8.47)%及其PD-1⁺的百分率(2.70±3.06)%与正常对照组(20.16±11.89)%和(0.43±0.36)%相比,差异有统计学意义(P<0.05)。结论:外周血CD4⁺CD25^highTreg数量下降及其PD-1分子表达上调可能在SLE中有重要意义。
- 红斑狼疮,系统性 /
- 调节性T细胞 /
- 程序性死亡受体-1
Abstract: Objective:To detect frequency of and programmed cell death-1(PD-1) expression of CD4⁺CD25^highTreg peripheral blood of systemic lupus erythematosus(SLE) patients and to explore their roles in immune disorder of SLE. Methods:Flow cytometry(FCM) was used to determine the frequencies of peripheral blood CD4⁺CD25^highTreg and CD4⁺CD25^lowT cells and the PD-1 expression on these cells in SLE patients and healthy normal controls. Results:The frequency of CD4⁺CD25^highTreg among CD4⁺Treg in peripheral blood of SLE patients group(0.63±0.31)% decreased significantly(P<0.01),compared with normal control group(2.07±0.74)%. The PD-1 expression on CD4⁺CD25^highTreg in SLE group(24.99±18.65)% was obviously higher than that in normal control groups(6.97±1.92)% (P<0.05). There were also was no significant differences in both the frequency of CD4⁺CD25^lowT cells and the PD-1 expression on these subset cells among two groups(P<0.05). Conclusions:Decreased number of CD4⁺CD25^highTreg and upregulated expression of PD-1 on these Treg may play an important role in the pathogenesis of SLE.
- lupus erythematosus,systemic /
- regulatory T cell /
- programmed death-1