survivin、caspase-3在脑胶质瘤的表达及suvivin ASODN对U251细胞的影响
The survivin and caspase-3 expression in brain glioma and effection of survivin antisense oligodexynucleotides on U251 cell
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摘要: 目的:观察脑胶质瘤患者脑组织survivin、caspase-3的表达情况及survivin ASODN对脑胶质瘤U251细胞survivin和caspase-3表达的影响,探讨两者与脑胶质瘤发病及预后的相关性。方法:免疫组化法检测脑胶质瘤患者survivin和caspase-3蛋白表达,RT-PCR检测脑胶质瘤组织survivin mRNA表达,以脑胶质瘤U251细胞为研究对象,转染不同剂量survivin ASODN(100nmol/L、300nmol/L、500nmol/L),并于培养不同时间(24h、48h、72h)后,RT-PCR检测U251细胞survivin和caspase-3的表达。结果:脑胶质瘤患者脑组织survivin蛋白高表达(阳性率69.6%),而caspase-3低表达(阳性率50.7%),且两者表达的阳性率与肿瘤的恶性程度有关。U251细胞在不同浓度survivin ASODN转染不同时间后,以500nmol/L survivin ASODN转染72h对U251 survivin mRNA的抑制率达62.06%,与对照组及不同浓度组间比较差异均有统计学意义(P<0.05~P<0.01)。免疫组化结果显示转染后survivin表达下调,而caspase-3表达上调。结论:脑胶质瘤患者脑组织高表达survivin蛋白,而低表达caspase-3蛋白,survivin ASODN可诱导U251细胞株survivin mRNA及蛋白表达下调,而caspase-3蛋白表达水平上调。Abstract: Objective: To observe the expression of survivin and caspase-3 in brain glioma and effect of survivin antisense oligodexynucleotides(ASODN) on expression of survivin and caspase-3 in U251 cells,and explore the relation of the expression of survivin and caspase-3 to the formation and clinical prognosis of brain glioma.Methods: Survivin and caspase-3 protein were detected by immounohistochemistry of glioma.The glioma cells,U251 were transferred with survivin ASODN(100 nmol/L,300 nmol/L,500 nmol/L),expression of surviving and caspase-3 of tumor cells were measured by RT-PCR and immounohistochemistry.Results: The positive rates of survivn and caspase-3 in glioma were 69.6% and 50.7%,respectively,compared with health control,the differences were significant(P<0.05-P<0.01).Survivin ASODN induced downregulation of the survivin mRNA in a time and dose-dependent manner,survivin ASODN also induced downregulation of the survivin protein and upregulation of the caspase-3 protein.Conclusions: Survivn upregulated and caspase-3 downregulated in glioma.Survivin ASODN can downregulate the expression of survivin mRNA and protein and upregulate the expression of caspase-3 protein.
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