Abstract: Objective: To investigate Klebsiella pneumoniae infection in neonatal ward and its drug resistance so as to provide basis for rational use of antibiotics. Methods: Klebsiella pneumonia isolated by ATBTM New biochemical identification system in neonatal ward from Jun. 2008 to Jun. 2010 and the drug resistance (determined by K-B) were analyzed retrospectively. Results: A total of 123 strains of Klebsiella pneumoniae were isolated from the samples. Of them,76 were isolated from sputum and throat swab specimens,18 from tracheal intubations,15 from blood samples,9 from the urine and 5 from other specimens. Klebsiella pneumoniae was highly resistant to cephalosporin antibiotics and sensitive to imipenem and cephalosporins containing enzyme inhibitor. The rate of extendedspectrum β-lactamases (ESBLs) producing strains in Klebsiella pneumoniae was 60.9%. Conclusions: Klebsiella pneumoniae in neonatal ward produces high ESBLs, and is highly resistant to commonly used antimicrobial, presenting multidrug resistance, which should arouse the attention of the clinicians. Imipenem and cephalosporin containing inhibitors can be selected as the first choice for treatment of neonatal Klebsiella pneumoniae.