慢性应激大鼠血清肿瘤坏死因子-α、白细胞介素-6的变化及其对心率和血压的影响

徐艳秋; 王洪巨

慢性应激大鼠血清肿瘤坏死因子-α、白细胞介素-6的变化及其对心率和血压的影响

Change of serum tumor necrosis factor-α and interleukin-6 and its influence on the heart rate and blood pressure of rats with chronic stress

摘要

摘要: 目的:观察慢性应激大鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的变化,以及其对血压、心率的影响。方法:选用健康雄性SD大鼠40只,随机分2组。对照组10只不给予任何刺激,实验组30只参照Katz方法制作慢性应激模型。采用ELISA测定每只大鼠血清TNF-α、IL-6的水平,并记录血压和心率。结果:与对照组比较,实验组大鼠旷场活动性降低(P < 0.01),血清IL-6、TNF-α水平显著增高(P < 0.05),血压增高、心率加快(P < 0.01)。结论:慢性应激影响大鼠的情感行为,可以升高大鼠血清TNF-α、IL-6水平,并影响血压、心率。

Abstract: Objective: To observe the changes of serum tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6),and their influence on the blood pressure and heart rates of rats by establishing models of chronic stress. Methods: Forty healthy male SD rats were randomly divided into two groups. Control group (n=10) were not given any incentives,and the experimental group (n=30) were established models of chronic stress with Katz method. Serum TNF-α and IL-6 levels of the rats were measured by enzyme-linked immunoassay,and the blood pressure and heart rates were recorded. Results: Compared with the control group,the open field activity of the rats with chronic stress decreased (P < 0.01),serum IL-6 and TNF-α levels increased (P < 0.05),the blood pressure was elevated and the heart rate speeded (P < 0.01). Conclusions: Stress may affect the emotional behavior,increase the serum IL-6 and TNF-α levels and influence the blood pressure and heart rates of the rats.

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