Abstract: Objective: To study the relationship between the expression downregulation or absence of metastasis suppressor gene Kiss-1 and clinicopathological parameters of non-small cell lung carcinoma (NSCLC).Methods: The expression of Kiss-1 was detected in the tumor and adjacent tissue in 68 paraffin sections of NSCLC by immunohistochemistry (EliVision methods),and the results were used to analyze the relationship with clinicopathological parameters.Results: The positive rate of Kiss-1 in tumor (82.4%) was significantly higher than that in adjacent tissue (66.2%) (P<0.05).The difference of positive rate of Kiss-1 was significant between group 1 (with metastasis of lymph node) and group 2 (without metastasis of lymph node).There was significant difference between the well differentiated,moderately differentiated group (82.5%) and the poorly differentiated,undifferentiated group (42.9%).Kiss-1 expression in the (Ⅰ+Ⅱ) stage (81.8%) was distinctly higher than that in the Ⅲ stage (37.5%) (P<0.01).Conclusions: In the patients of NSCLC,the expression of Kiss-1 is lower in the group with metastasis of lymph node,poor differentiation and advanced stage,which may provide a theoretical basis of gene therapy for NSCLC.