Abstract: Objective: To investigate the distribution of pathogenic bacteria and its drug sensitivity of chronic suppurative otitis media,and guide clinical rational drug use.Methods: The data of the pus cultivation and drug sensitivity test of chronic suppurative otitis media in 156 patients were retrospectively analyzed.Results: One hundred and twenty-seven strains pathogenic bacteria were detected in 156 specimens,which included 119 strains of bacteria and 8 strains of fungi.The four leading bacteria were coagulase negative staphylococcus(41 strains,32.23%),pseudomonas aeruginosa(19 strains,15.0%),staphylococcus aureus(18 strains,14.2%) and proteus(11 cases,8.7%).Different pathogenic bacteria had different drug sensitivities.Conclusions: The coagulase negative staphylococcus,pseudomonas aeruginosa,staphylococcus aureus and proteus are the major pathogenic bacteria in chronic suppurative otitis media.The reasonable choice of antibiotics should base on the drug sensitive test.