PCR检测耐甲氧西林凝固酶阴性葡萄球菌耐药基因

Detection of drug-resistant genes in meticillin-resistant coagulase negative staphylococcus by PCR assay

摘要: 目的: 了解安徽省宿州地区临床分离的耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)所携带的7种耐药基因状况。方法: 使用聚合酶链反应方法检测133株MRCNS中mecA、gyrA、qacA/B/C、qacA、ermA/B/C、ermB、TetM耐药基因。结果: 133株MRCNS中,mecA基因携带率为100.0%,gyrA、qacA/B/C、qacA、TetM、ermA/B/C、ermB基因携带率依次为47.4%、51.9%、45.1%、25.6%、24.8%、12.0%。结论: 安徽省宿州地区MRCNS中mecA、qacA/B/C、qacA基因携带情况常见,gyrA、TetM基因携带率较高,而ermA/B/C、ermB较低。

Abstract: Objective: To investigate the seven drug-resistance genes in meticillin-resistant coagulase negative staphylococcus (MRCNS) in Suzhou area. Methods: The drug-resistance genes of mecA,gyrA,qacA/B/C,qacA,ermA/B/C,ermB and TetM were determined with polymerase chain reaction in 133 strains of MRCNS. Results: In the 133 strains of MRCNS,the carrier rates of mecA,gyrA,TetM,qacA/B/C,ermB,qacA and ermA/B/C were 100.0%,47.4%,51.9%,45.1%,25.6%,24.8% and 12.0%,respectively. Conclusions: In Suzhou area of Anhui province,the carrier rates of mecA,qacA/B/C and qacA genes in MRCNS are similar to that of other domestic regions,but that of gyrA,and TetM genes are higher,and ermA/B/C and ermB lower.