miR-223-3p通过靶向TGFBR3促进LncRNA ADAMTS9-AS2表达上调抑制肺癌细胞的增殖和迁移作用

陈平; 张鹤; 李少军

doi:10.13898/j.cnki.issn.1000-2200.2024.01.004

miR-223-3p通过靶向TGFBR3促进LncRNA ADAMTS9-AS2表达上调抑制肺癌细胞的增殖和迁移作用

miR-223-3p promotes the up-regulation of LncRNA ADAMTS9-AS2 expression by targeting TGFBR3 to inhibit the proliferation and migration of lung cancer cells

摘要

摘要:
目的探讨miR-223-3p通过靶向转化生长因子-βⅢ型受体（TGFBR3）促进长链非编码RNA（LncRNA）ADAMTS9-AS2表达上调抑制肺癌细胞增殖和迁移的作用及可能机制。
方法采用RT-PCR检测肺癌H1299细胞中miR-223-3p和TGFBR3 mRNA表达水平，Western blotting检测TGFBR3的蛋白表达水平，RT-qPCR检测LncRNA ADAMTS9-AS2的表达水平，CCK-8检测细胞增殖能力，Transwell细胞迁移实验和划痕实验检测细胞迁移能力。采用脂质体转染miR-223-3p模拟物（过表达组）、抑制剂（抑制组）、对照质粒（对照组）于H1299细胞中，比较各组转染前后miR-223-3p、TGFBR3、LncRNA ADAMTS9-AS2表达水平、细胞增殖能力、细胞迁移能力。
结果与转染前比较，过表达组转染后的H1299细胞中miR-223-3p、LncRNA ADAMTS9-AS2表达水平均升高（P < 0.05），TGFBR3蛋白和mRNA表达水平均降低（P < 0.01和P < 0.05），细胞增殖和迁移能力下降（P < 0.05）；抑制组转染后的细胞中miR-223-3p和LncRNA ADAMTS9-AS2表达水平均降低（P < 0.05），TGFBR3蛋白和mRNA表达水平均升高（P < 0.01和P < 0.05），细胞增殖和迁移能力均增加（P < 0.05）。转染72 h后，TGFBR3蛋白表达水平及mRNA的表达水平细胞增殖与迁移能力：抑制组>观察组>过表达组（P < 0.01）。3组miR-223-3p、LncRNA ADAMTS9-AS2 mRNA表达水平逐渐降低，抑制组<对照组<过表达组（P < 0.01）。
结论miR-223-3p抑制肺癌H1299细胞的增殖和迁移，靶向下调TGFBR3和上调LncRNA ADAMTS9-AS2表达可能为其作用机制。

Abstract:
ObjectiveTo investigate the effect of miR-223-3p on the up-regulation of long non-coding RNA(LncRNA) ADAMTS9-AS2 by targeting transforming growth factor-β type Ⅲ receptor(TGFBR3) to inhibit the proliferation and migration of lung cancer cells.
MethodsThe mRNA expression levels of miR-223-3p and TGFBR3 in lung cancer H1299 cells were detected by RT-PCR, the protein expression level of TGFBR3 was detected by Western blotting, the expression level of LncRNA ADAMTS9-AS2 was detected by RT-qPCR, the cell proliferation ability was detected by CCK-8, and the cell migration ability was detected by Transwell migration assay and scratch assay.MiR-223-3p mimic(overexpression group), inhibitor(inhibition group) and control plasmid(control group) were transfected into H1299 cells by liposome.The expression levels of miR-223-3p, TGFBR3, LncRNA ADAMTS9-AS2, cell proliferation and cell migration were compared between before and after transfection.
ResultsCompared with before transfection, the expression levels of miR-223-3p mRNA and LncRNA ADAMTS9-AS2 in H1299 cells after transfection in the overexpression group increased(P < 0.05), the expression levels of TGFBR3 protein and mRNA decreased(P < 0.01 and P < 0.05), and the cell proliferation and migration ability decreased(P < 0.05);the expression levels of miR-223-3p mRNA and LncRNA ADAMTS9-AS2 in the inhibition group decreased(P < 0.05), the expression levels of TGFBR3 protein and mRNA increased(P < 0.01 and P < 0.05), and the cell proliferation and migration ability increased(P < 0.05).After 72 h of transfection, the expression level of TGFBR3 protein and mRNA, and the cell proliferation and migration ability were as follows: inhibition group>observation group>overexpression group(P < 0.01).The expression levels of miR-233-3p and LncRNA ADAMTS9-AS2 mRNA gradually decreased, Which were as follows: inhibition group<control group<overexpression group(P < 0.01).
ConclusionsMiR-223-3p inhibits the proliferation and migration of lung cancer H1299 cells, the mechanism of which may involve the targeted down-regulation of TGFBR3 and up-regulation of LncRNA ADAMTS9-AS2 expression.

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