Abstract: Objective: To study the general characteristics and antibiotic resistance of Staphylococcus aureus isolated from various locus of infection and to guide antibiotic chemotherapy in clinics.Methods: The pathogenic bacteria isolated from various locus of infection were identified with Vitek-32 automatic microbial monitor and performed antimicrobial sensitivity test.Results: One hundred sixteen strains of Staphylococcus aureus were isolated,and the age of inpatients was much higher than that of outpatients(P<0.05).The Staphylococcus aureus isolated from the respiratory infection of the inpatients were tested with 14 antibacterials,and the antimicrobial agents with a resistant rate over 60% were penicillin G,ampicillin,erythromycin,cefazolin,levofloxacin,gentamycin,tetraycline,terimethoprim-sulfamethoxzole and clindamycin.The Staphylococcus aureus isolated from respiratory infection of inpatient had a higher resistant rate,detection rate and multidrug resistance than the strains isolated from other locus of infecion(P<0.05).The Staphylococcus aureus isolated from skin and soft tissue infection of outpatient had a higher resistance to multidrug antimicrobial agents,higher detection rate of MRSA than the strains islated from other locus of infecion of inpatients(P<0.05).Conclusions: Staphlococcus aureus isolated from the respiratory infection of inpatient and the skin and soft tissue infection of outpatients were different from strains isolated from other locus of infection of inpatients,and use of antibiotics should be different.Staphylococcus aureus isolated from the respiratory infection of inpatients were highly resistant to most of the antimicrobial agents and demonstrated a higher detection rate of MRSA.Monitoring of drug resistance should be strengthened to prevent the spread and epidemic of the resistant strains.