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    As2O3诱导人膀胱癌BIU-87细胞株凋亡及其对细胞周期的影响

    Arsenic trioxide induced apoptosis in human bladder cancer BIU-87 cell line and its influence on cell cycle

      摘要
    • 摘要: 目的: 观察三氧化二砷(As2O3)对人膀胱癌BIU-87细胞株生长抑制作用,并探讨对细胞周期及凋亡的影响。方法: CellTiter 96 Aqueous One试剂检测BIU-87细胞株增殖活力;荧光染色观察凋亡细胞的形态学变化;流式细胞术分析细胞周期及凋亡。结果: As2O3与BIU-87细胞株生长抑制率之间有明显的浓度依赖关系(P<0.01),I C50为28.92 μmol/L;荧光显微镜下观察到经As2O3作用的BIU-87细胞核膜消失,核染色质呈大小不等的小圆形碎片;流式细胞术结果表明30 μmol/L的As2O3处理BIU-87细胞株48 h后,G0/G1期细胞比例下降,S期细胞比例增加,凋亡率为47.37%。结论: As2O3能显著抑制BIU-87细胞株增殖;使细胞阻滞在S期,并进一步诱导细胞凋亡。

       

      Abstract: Objective: To observe the effects of arsenic trioxide (As2O3) on the cell growth,cell cycle and apoptosis of human bladder cancer BIU-87 cells line.Methods: CellTiter 96 Aqueous One was used to determine the cell proliferation.Morphological changes of BIU-87 cells were observed by fluorescence staining of Hoechst 33258.Cell cycle and apoptosis were analyzed by flow cytometry (FCM).Results: There was obviously a concentration-dependent relationship between As2O3 and the inhibition of BIU-87 cell proliferation(P<0.01).IC 50 was 28.92 μmol/L.Morphological observation by fluorescence staining indicated that As2O3 induced apoptosis of BIU-87 cells.FCM demonstrated that the number of cells in G0/G1 phase was decreased,while that in S phase were increased after treated with As2O3,and apoptosis rate was 47.37%.Conclusions: The results suggest that As2O3 can inhibit human bladder cancer cells BIU-87 growth by means of cell proliferation inhibition,S phase arrest and induction of apoptosis. Back

       

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