Abstract: Objective: To explore the expression of micro RNA(miRNA)-143 in non-small cell lung cancer(NSCLC) tissue and relationship with clinicopathological features of tissue.Methods: The expression levels of miRNA-143 in NSCLC and adjacent cancerous tissue of 40 cases were detected by real-time PCR.The relationship of miRNA-143 expression with clinicopathological features of tissue was analyzed.Results: The expression of miRNA-143 in NSCLC tissue was significantly lower than that in adjacent cancerous tissue(P<0.05).The expression differences of miRNA-143 in different ages,gender,histologic types,differentiation degrees and TNM stages were not statistical significance(P>0.05).The expression level of miRNA-143 in NSCLC tissue with lymph node metastasis was lower than that in NSCLC tissue without lymph node metastasis(P<0.05).Conclusions: The expression level of miRNA-143 in NSCLC tissue decreases,which may be correlated with the cell metastasis of lung cancer.