改性聚乳酸与Schwann细胞的生物相容性研究

Study of the biocompatibility of modified polylactic acid vector with Schwann cell

摘要: 目的:对不同的改性聚乳酸材料进行Schwann细胞(SC)生物相容性观察。方法:采用细胞增殖度法,观察不同材料浸提液对SC增殖影响,进行毒性评级;采用相差显微镜、扫描电镜、荧光分光光度法观察细胞形态以及黏附情况。结果:除2^#材料(PLA-g-HEMH)的高浓度浸提液毒级为2级外,其余毒级均为0~1级,细胞生长良好,其黏附性较未改性聚乳酸高,但所有材料SC的黏附性仍低于培养板(P<0.01)。结论:改性后的材料细胞生物相容性良好,其表面亲水性增加,但2^#材料可能在改性过程中表面酸度增加,在处理上有必要改进。

Abstract: Objective: To observe the biocompatibility of modified polylactic acid vector with Schwann cell.Methods: Immersed extraction of modified PLA affect to Proliferation and cytotoxicity stage of Schwann cell by MTT method,Schwann cell morphology and adhesion condition on surface of materials were observated by scanning electro microscope and Fluor spectrophotometer.Results: Cytotoxicity stage of modified polylactic acid vector were 0-1 stage,and the mumble of schwann cells and adhesion were better than unmodified PLA,but all materials were as good as culture well(P< 0.01).Conclusions: Modified polylactic acid vector have better biocompatibility with Schwann cell,and their cytotropism were improved.