革兰阴性菌L型与其原菌内毒素含量比较

Comparison of endotoxin content between Gram negative bacteria and their L-forms

摘要: 目的: 探讨革兰阴性菌L型和原菌内毒素含量差异。方法: 采用定性和定量鲎试验,分别检测稳定的大肠埃希菌和铜绿假单胞菌L型及其原菌内毒素含量。结果: 大肠埃希菌和铜绿假单胞菌L型内毒素定性鲎试验检测阳性,定量鲎试验检测出大肠埃希菌和铜绿假单胞菌L型内毒素含量分别为原菌内毒素含量的25.1%和36.4%。结论: 革兰阴性菌L型仍能产生内毒素,但其含量较原菌明显减少,是其致病力减弱的原因之一。

Abstract: Objective: To compare endotoxin content between Gram negative bacteria and their L-forms.Methods: The endotoxin content was detected by qualitative and quantitative limulus tests in E.coli and P.aeruginosa and their stable L-forms.Results: The qualitative limulus tests of Gram negative bacterial L-forms were positive.The endotoxin content of E.coli L-forms is 25.1% of their parental bacteria,and P.aeruginosa L-forms was 36.4% of parental.Conclusions: Gram negative bacterial L-forms generate less endotoxin.