Abstract: Objective: To observe the barrier of wood pulp spunlace against bacteria.Methods: One to four layers of wood pulp spunlace or white cotton cloth from market were put on the surface of culture medium plate.Suspension of E.coli and S.aureus at different dilution were dropped on the dried or wet cloths.After culture,the numbers of bacteria colonies were counted.Results: One layer of dried or wet wood pulp spunlace were able to,but white cotton cloth from market did not,prevent permeation from bacteria.Only four layers of dried white cotton cloth from market were able to prevent the permeating from the bacteria.Conclusions: Wood pulp spunlace had a better barrier function against bacteria than that of white cotton cloth from market.