N-乙酰半胱氨酸对小鼠肺腺癌生长、转移的抑制作用

    Role of N-acetylcysteine in inhibiting the growth and metastasis of lung adenocarcinoma in mice

    • 摘要: 目的: 探讨N-乙酰半胱氨酸(NAC)对T739小鼠肺腺癌原发瘤形成及自发性肺转移的影响。方法: T739小鼠51只随机分为3组,其中Ⅰ组为对照组,Ⅱ、Ⅲ组分别在每天饮水中加入0.5g/kg、1.0g/kgNAC,72h后皮下接种小鼠LA795肺腺癌细胞(0.2×10P>6P>个/只),观察小鼠体重变化、皮下肿瘤形成、生长及肺转移情况;采用二硫代对硝苯甲酸显色法测定血中谷胱甘肽(GSH)含量。结果: 接种第4天,肿瘤出现率Ⅱ、Ⅲ组分别为47.1%和38.9%,均明显低于Ⅰ组81.25%(P<0.05)。第22天处死小鼠后皮下肿瘤重量Ⅱ、Ⅲ组明显低于Ⅰ组(P<0.05和P<0.01),且与NAC剂量呈负相关(P<0.01)。各组之间自发性肺转移灶的数目差异均无显著性(P>0.05)。各组小鼠体重实验后均较实验前明显下降(P<0.01),但实验后3组之间体重差异均无显著性(P>0.05)。血GSH含量Ⅰ组明显低于Ⅱ、Ⅲ组(P<0.01)。结论: NAC可明显延长肿瘤形成的潜伏期,抑制原发瘤的生长;NAC对小鼠无明显副作用,是一种安全可靠的制剂。

       

      Abstract: Objective: To study the influence of N-acetylcysteine(NAC) on the growth and spontaneous metastasis of lung adenocarcinoma in T739 mice.Methods: Fifty-one T739 mice were randomly divided into 3 groups.Group Ⅰ acted as the control.Group Ⅱ and Ⅲ were administered different doses of NAC(0.5 g·kg-1·d-1,1.0 g·kg-1·d-1) in drinking water 72 hours before subcutaneous inoculation of LA795 lung adenocarcinoma cells(0.2×106),and then the formation and growth of the primary tumor,the number of lung metastasis and the concentration of glutathione(GSH)were observed.Results: On the fourth day after inoculation,the incidence of tumor was 81.25% in the control group,which was much higher than that of the treatment groups(47.1%,38.9%,respectively)(P<0.05).The weight of the tumor decreased significantly(P<0.05 and P<0.01) compared with the control group.The doses of NAC was negatively related to the weight of the local tumor(P<0.01).The influence of NAC on the number of lung metastasis was not statistically significant(P>0.05).The weight of the mice decreased greatly(P<0.01) compared to the initial weight in all the groups and the difference between the 3 groups was not significant(P>0.05).Conclusions: NAC can inhibit the formation of the local tumor and has no significant side-effects on the mice.It is a promising agent.

       

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