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    芦笋提取液抑制恶性黑色素瘤A375细胞增殖的研究

    Asparagus extract in inhibiting the proliferation of melanoma A375 cells

      摘要
    • 摘要: 目的: 探讨芦笋提取液对人恶性黑色素瘤A375细胞增殖的抑制作用以及对其凋亡的诱导作用。方法: 用四甲基噻唑氮蓝比色法测定A375细胞活力;流式细胞术(FCM)检测A375细胞的凋亡诱导及杀伤作用(P<0.01)。结果: 芦笋提取液与人恶性黑色素瘤细胞株A375细胞生长抑制率之间有明显的浓度依赖关系,浓度分别为200 mg/ml、400 mg/ml、600 mg/ml和800 mg/ml时,A375细胞的凋亡率及坏死率分别为2.39%、8.85%、8.71%、5.16%和1.65%、1.51%、22.10%、82.80%。结论: 芦笋提取液能显著抑制恶性黑色素瘤A375细胞的增殖,且诱导A375细胞总的凋亡率和坏死率与其浓度之间存在着依赖关系。

       

      Abstract: Objective: To explore the effects of asparagus extract on the inhibition of proliferation and apoptosis in human melanoma A375 cells.Methods: The cell proliferation inhibition was measured by methyl thiazolyl tetrazolium(MTT) colorimetric assay;apoptosis and necrosis in A375 cells were analysed by flow cytometry.Results: There was obviously a concentration-dependent relationship between the asparagus extract and the inhibition of A375 cells proliferation(P<0.01).The rates of apoptosis and necrosis in A375 cells were 2.39%, 8.85%,8.71%,5.16% and 1.65%,1.51%,22.10%,82.80% respectively as treated at doses of asparagus extract 200 mg/ml,400 mg/ml,600 mg/ml and 800 mg/ml.Conclusions: Asparagus extract can obviously inhibit the proliferation of A375 cells.There exists a concentration dependent relationship between asparagus extract and the total rates of apoptosis and necrosis.

       

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