10-羟基喜树碱联合热疗对肝癌协同杀伤作用的研究

祝子华; 祝金泉

10-羟基喜树碱联合热疗对肝癌协同杀伤作用的研究

Synergic effect of the combination of thermotherapy and 10-hydroxycamp-tothecine(HCPT) on hepatoma cells in vitro

摘要

摘要: 目的: 研究10-羟基喜树碱(HCPT)联合热疗对肝癌细胞有无协同杀伤作用,为临床应用提供实验依据。方法: 人肝癌细胞株SMMC-7721进行细胞培养、传代,实验分成四组:(1)单纯热疗组;(2)单纯HCPT组;(3)热疗与HCPT联合组;(4)对照组,各组分别进行实验,用MTT法测定各组对细胞的杀伤作用。结果: 单纯热疗显示温度43℃、44.5℃(各30 min)对SMMC-7721细胞有明显的杀伤作用(P<0.01),而40℃、41.5℃(各30 min)对细胞杀伤作用不明显(P>0.05)。HCPT对肝癌SMMC-7721细胞有明显杀伤作用(P<0.01),呈时间依赖性和浓度依赖性(P<0.01)。HCPT联合热疗对细胞的杀伤作用明显强于单纯的HCP T或热疗的作用(P<0.01)。结论: HCPT联合热疗对肝癌细胞的杀伤有明显的协同作用。

Abstract: Objective: To investigate whether the combination of thermotherapy and HCPT has a synergic effect on the hepatoma cells(SMMC-7721 cell line) in vitro.Methods: The experiment consists of four groups:thermotherapy,chemotherapy(HCPT),thermo-chemotherapy(chermotherapy combined with HCPT) and control group.Results: Thermotherapy within 30 minutes with a temperature of 43℃,44.5℃ had an obviously inhibited SMMC-7721 cells(P<0.01),while 40℃,41.5℃ hardly had any effect on the cells(P>0.05).HCPT had a great cytotoxicity effect on SMMC-7721 cells in dose and time-dependant manner(P<0.01).The cytotoxicity could be enhanced when HCPT was combined with thermotherapy(P<0.01).Conclusions: The combination of HCPT and themotherapy has a remarkable synergic effect on hepatoma cells.

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