浸水急性冷应激对大鼠脑皮质神经元一氧化氮释放量的影响

Effect of acute cold stress by immersing in water on the release of nitric oxide in cerebral cortex of rats

摘要: 目的: 探讨浸水急性冷应激对大鼠脑皮质神经元一氧化氮(nitricoxide,NO)释放量的影响。方法: 将27只健康、成年、雄性Wistar大鼠随机分为A、B、C组。A组为对照组,不作任何处理;B组为浸水急性冷应激30 min组;C组为浸水急性冷应激60 min组。大脑皮质NO含量使用硝酸酶还原法试剂盒测定。结果: A组大鼠脑皮质NO含量与B组差异无显著性(P>0.05),而C组大鼠脑皮质NO含量均明显高于A组和B组(P<0.01)。结论: 一氧化氮在急性冷应激早期或许对脑皮质神经元有保护作用。

Abstract: Objective: To elucidate the changes of nitric oxide production in cerebral cortex of rats under acute cold stress by immersing in water.Methods: Twenty-seven male adult rats were randomly divided into three groups.Group A was a control group and rats in which were without any treatment.Rats of group B were put into cold water for 30 minutes and rats of group C were put into cold water for 60 minutes.The level of nitric oxide in cortex of rats were deteated by using NO deteat Kit.Results: The levels of nitric oxide in group A,B,and C were(4.36±1.79) mmol/g pr;(2.79±2.00) mmol/gpr,and(11.50±2.34) mmol/gpr,respectively.Conclusions: Nitric oxide may play a protective role in the early stage of acute cold stress.