尿N-乙酰-β-D-氨基葡萄糖苷酶联合尿微量蛋白检测诊断高血压患者早期肾损伤

朱晴; 程训民; 李朋

尿N-乙酰-β-D-氨基葡萄糖苷酶联合尿微量蛋白检测诊断高血压患者早期肾损伤

Significance of detecting urine micralbumin and n-acetyl-β-D-glucosaminidase in diagnosis of early renal damage in hypertensive patients

摘要

摘要: 目的:探讨尿N-乙酰-β-D-氨基葡萄糖苷酶(NAG酶)联合尿微量白蛋白(mALB)检测对诊断高血压早期肾损伤的价值。方法:对78例高血压患者应用干化学试剂法测定尿常规蛋白,速率散射免疫比浊法测定尿mALB,琼脂糖凝胶电泳法测定尿NAG酶的活性;选择38名健康体检者设为对照组。结果:高血压患者尿mALB阳性率为55.13%,明显高于尿常规蛋白的12.82%(P<0.01);高血压患者尿NAG及mALB均明显高于对照组(P<0.01),并随病程延长有逐渐增高趋势。结论:尿mALB和尿NAG酶联合检测对高血压患者的早期肾损伤有指导价值。

Abstract: Objective:To investigate the value of detecting microalbumin(mALB) and n-acetyl-β-D-glucosaminidase(NAG) in diagnosis of early renal damage in patients with hypertension.Methods:Mimmunoprecipitation in liquid phase was used to measure the urinary mALB,and the rate method was employed to determine the urinary NAG activity of 78 patients with hypertension,apply agarose gel electrophoresis detect vrine NAG activated.Results:The mALB in 43 cases(55.13%) increased,which was significantly higher than that of conventionl urine protein positive rate of 12.82(P<0.01).The urinary NAG and urinary mALB in the patients with hypertension were significantly higher than those of the control(P<0.01),and tended to rise with the duration of the disease.Conclusions:Urinary mALB and urinary NAG may help to predict the hypertensive renal damage in the early stage.

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