吴礼高, 何杰, 王朝夫, 吴强, 龚晓萌. 胶质瘤p53、p16、bcl-2、c-fos蛋白表达和细胞凋亡[J]. 蚌埠医学院学报, 2006, 31(6): 572-574.
    引用本文: 吴礼高, 何杰, 王朝夫, 吴强, 龚晓萌. 胶质瘤p53、p16、bcl-2、c-fos蛋白表达和细胞凋亡[J]. 蚌埠医学院学报, 2006, 31(6): 572-574.
    WU Li-gao, HE Jie, WANG Chao-fu, WU Qiang, GONG Xiao-meng. Expression of p53,p16,bcl-2,c-fos protein and cell apoptosis in 150 cases of cerebral glioma[J]. Journal of Bengbu Medical College, 2006, 31(6): 572-574.
    Citation: WU Li-gao, HE Jie, WANG Chao-fu, WU Qiang, GONG Xiao-meng. Expression of p53,p16,bcl-2,c-fos protein and cell apoptosis in 150 cases of cerebral glioma[J]. Journal of Bengbu Medical College, 2006, 31(6): 572-574.

    胶质瘤p53、p16、bcl-2、c-fos蛋白表达和细胞凋亡

    Expression of p53,p16,bcl-2,c-fos protein and cell apoptosis in 150 cases of cerebral glioma

    • 摘要: 目的: 探讨p53、p16、bcl-2、c-fos基因在胶质瘤的表达水平和细胞凋亡与胶质瘤的发生、发展的关系。方法: 采用免疫组织化学和原位末端标记(ISEL)技术,对不同组织学级别150例胶质瘤石蜡标本进行基因蛋白和细胞凋亡的检测。结果: 150例胶质瘤中p53、p16、bcl-2、c-fos蛋白的阳性率分别为50.00%、38.67%、61.33%、46.67%;p53、p16、bcl-2蛋白表达差异在各组间均有统计学意义(P<0.005),c-fos蛋白表达差异无统计学意义(P>0.05);随胶质瘤级别升高,bcl-2蛋白表达水平增高,瘤细胞凋亡减少,但差异无显著性(P>0.05)。结论: p53、p16、bcl-2、c-fos基因的异常表达在胶质瘤的发生、发展过程中起重要作用。p53、p16、bcl-2、c-fos基因的协同作用促使细胞凋亡机制障碍,细胞增殖和凋亡失控。p53、p16、bcl-2、c-fos基因蛋白的检测对判断预后、指导临床基因治疗有重要意义。

       

      Abstract: Objective: To investgate the relationship between p53,p16,bcl-2,c-fos gene expression level and cell apoptosis in cerebral glioma with the development and progression.Methods: The expression of p53,p16,bcl-2,c-fos protein was detected in 150 cases of different grade cerebral glioma by immunohistochemistry and cell apoptosis by using in situ end labeling(ISEL) technique.Results: The positive expression rate of p53,p16,bcl-2,c-fos protein in 150 cases of cerebral glioma was 56.67,38.67,61.37, 46.67,respectively;There was significant difference of the expression of p53,p16,bcl-2 protein among Ⅰ-Ⅳgrade glioma (P<0.005),there was no significant difference of the expression of c-fos protein(P>0.05).With the higher of the positive intensity of bcl-2 protein,cell apoptosis was decrease in the tumor,but there was no significant difference of the cell apoptosis (P>0.05).Conclusions: The abnormal expression of p53,p16,bcl-2,c-fos protein played an important role in the development and progression of cerebral glioma.The cooperation of the p53,p16,bcl-2,c-fos protein may cause disorder of cell apoptosis,and unbalance of cell proliferation and apoptosis.Then the detection of p53,p16,bcl-2,c-fos protein may play an important role in prognosis and clinic gene therapy.

       

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