Abstract: Objective:To study the expression levels and clinical significance of secretory crimp(sFRP1) and β-catenin in Wnt signaling pathway,and its correlation with HPV infection.Methods:The expression levels of sFRP1 and β-catenin protein in 60 cervical squamous cell carcinoma(SCC) cases,75 cervical intraepithelial neoplasia cases(including 15 cases of LSIL and 60 cases of HSIL) and 30 normal cervical tissue cases were detected using immunohistochemical SP method,and the HPV-DNA of cervical mucus exfoliated cells was detected using the hybrid capture(HC₂) technology.Results:The expression levels of sFRP1 protein in cytomembrane of normal cervical tissue,cervical intraepithelial neoplasia and cervical squamous carcinoma gradually decreased,and the expression levels of β-catenin protein in cytoplasm and nucleus of normal cervical tissue,cervical intraepithelial neoplasia and cervical squamous carcinoma gradually increased.The differences of sFRP1 expression in SCC tissue with different pathological differentiation and infiltration depth were statistically significant(P<0.05),and the differences of β-catenin expression in SCC tissue with different FIGO stages and infiltration depth were statistically significant(P<0.05).In SCC tissue,the expression of β-catenin was negatively correlated with the expression of sFRP1(P<0.05),the expression of β-catenin was negatively correlated with HPV infection(P<0.01),and the expression of sFRP1 was negatively correlated with HPV infection(P<0.05).Conclusions:The combined detection of the expression of sFRP-1 and β-catenin,and HPV infection has important significance in judging the occurrence,development and prognosis of cervical cancer.