Abstract: Objective:Developing a computer software for analysis of arterial blood gases for clinical and teaching use,which can analog display the process of diagnosis and analysis. Methods:The software was edited by means of elanguage and combined with Windows operating system. Results:The software accurately determined different acid-base imbalances and respiratory dysfunction. The software analog displayed that the process of diagnosis and analysis of blood gas analysis by the six-step. After determining the type of acid-base imbalance,the software provided the incidence of the causes,clinical manifestations and treatment recommendations of it for clinicians reference. Conclusions:The software can be used as a reference by clinical doctors in diagnosis and analysis of blood gas analysis,it could also be used for teaching and training. It is scientific,advanced and valuable in clinical practice.