Abstract: Objective: To observe the Gram-negative bacterial resistance in patients in Intensive Care Unit(ICU) and to discuss how to treat infections caused by these resistant strains.Methods: The minimal inhibitory concentrations of 10 antibiotics against 160 strains of Gram-negative bacilli isolated from ICU patients were determined by E test.Results: The resistance rates to imipenem,cefepime and sulbactam-cefoperazone were the lowest,being 11.3%、18.8% and 20.6%,respectively.Forty-one ESBLs-producing strains were susceptible to imipenem,cefepime and sulbactam-cefoperazone,with a susceptible rate of 90.2%,82.9% and 80.5%,respectively.Conclusions: Imipenem is the most active agent against severe systemic infections caused by ESBLs-producing strains,while β-lactam/β-lactamase inhibitors are also active against most strains.