Abstract: Objective: To reconstruct and visualize three-dimentional model of Rolando fissure for the stereotactic anatomy.Methods: Rolando fissure,the lateral ventricle,the cerebrum and the borderyline of head were identified and segmented on a series of transverse MRI of head,and the three-dimentional model of them were reconstructed in 3D-Doctor workstation with the method of surface rendering.Results: The three-dimentional model of Rolando fissure,other interesting structures and their spatial relation were observated in three-dimensional virtual space in random angles.Conclusions: Anatomical observation and measurement can be implemented in the three-dimentional model.Furthermore,it was hoped that the model would benefit to virtual surgical training and surgical planning in neurosurgery.