Abstract: Objective: To establish the model of immunological liver injury in mice and investigate the related mechanisms.Methods: Twenty mice were randomly divided into normal control and Con A groups,10 in each.Mice of Con A group were injected with Con A 2.5 mg·ml^-1·kg^-1 by vena caudalis,and sacrificed after 8 hours.The blood and liver tissue were collected to examine ALT and AST levels spectrophotometrically,the histopathology changes were measured by HE method,and serum TNF-α and IFN-γ contents were measured by ELISA.Results: Compared with normal control group,serum ALT and AST levels were significantly elevated,serum TNF-α and IFN-γ contents were markedly increased(P<0.01).Meanwhile,hepatic necrosis and neutrophil infiltration also occured in Con A group.Conclusions: The model of immunological liver injury in mice was established by vena caudalis injection of Con A,the related mechanism is partially due to regulating the expressions of inflammatory cytokines.