低剂量阿糖胞苷对Jurkat T淋巴细胞CD147表达的影响

The effect of Ara-C at low concentrations on CD147 expression in Jurkat cells

摘要: 目的: 探讨低剂量阿糖胞苷(Ara-C)对Jurkat T淋巴细胞CD147表达的影响。方法: 实验细胞分6组,为对照组和1、5、10、20、30 ng/ml Ara-C组。采用RT-PCR、Western blot和流式细胞术分别检测Ara-C干预前后Jurkat细胞CD147基因和蛋白的表达。用倒置显微镜观察、比较Ara-C干预前后Jurkat细胞集落形成情况。结果: Ara-C各浓度组中细胞CD147 mRNA和蛋白的表达均明显高于对照组(P < 0.01),Ara-C干预组细胞集落形成明显增加。结论: 低剂量Ara-C可上调Jurkat T淋巴细胞CD147的表达,增强细胞间黏附力。

Abstract: Objective: To investigate the effect of Ara-C at low concentrations on extracellular matrix metalloproteinase inducer (CD147) expression in Jurkat cells. Methods: Jurkat cells were divided into six groups:control group and cells treated with different concentrations of Ara-C at 1,5,10,20,and 30 ng/ml. CD147 mRNA and protein expression were assayed by RT-PCR,Western blot and flow cytometry,respectively. The colony formation in Jurkat cells which was stimulated with Ara-C or not was observed by inverted microscope. Results: Different concentrations of Ara-C significantly up-regulated the expressions of CD147 mRNA and protein,and increased the colony formation in Jurkat cells (P < 0.01). Conclusions: Ara-C at low concentrations can enhance the expression of CD147 and increase the adhesive force in Jurkat cells.