任汝静, 王刚, 周海燕, 陆国强, 陈生弟. 鱼藤酮及神经肽PACAP27对PC12细胞PAC1受体及内源性PACAP表达的影响[J]. 蚌埠医学院学报, 2007, 32(6): 631-632,636.
    引用本文: 任汝静, 王刚, 周海燕, 陆国强, 陈生弟. 鱼藤酮及神经肽PACAP27对PC12细胞PAC1受体及内源性PACAP表达的影响[J]. 蚌埠医学院学报, 2007, 32(6): 631-632,636.
    REN Ru-jing, WANG Gang, ZHOU Hai-yan, LU Guo-qiang, CHEN Sheng-di. Effects of gene expression of endogenous PACAP and PAC1 receptor in PC12 cells under administration of rotenone and neuropeptide PACAP[J]. Journal of Bengbu Medical College, 2007, 32(6): 631-632,636.
    Citation: REN Ru-jing, WANG Gang, ZHOU Hai-yan, LU Guo-qiang, CHEN Sheng-di. Effects of gene expression of endogenous PACAP and PAC1 receptor in PC12 cells under administration of rotenone and neuropeptide PACAP[J]. Journal of Bengbu Medical College, 2007, 32(6): 631-632,636.

    鱼藤酮及神经肽PACAP27对PC12细胞PAC1受体及内源性PACAP表达的影响

    Effects of gene expression of endogenous PACAP and PAC1 receptor in PC12 cells under administration of rotenone and neuropeptide PACAP

    • 摘要: 目的: 探讨神经毒素鱼藤酮及神经肽PACAP27作用大鼠嗜铬细胞瘤细胞(PC12细胞)后,PC12细胞自身PAC1受体及自身内源性垂体腺苷酸环化酶激活肽(PACAP)的表达变化。方法: 采用定量逆转录多聚酶链反应(RT-PCR)检测PC12细胞PAC1受体和PACAP mRNA表达。结果: 鱼藤酮(250 nmol/L)可使PAC1受体mRNA的表达增高;神经肽PACAP单独作用或与鱼藤酮和(或) PAC1受体拮抗剂PACAP 6-27联合作用时均对PAC1受体的表达无影响(P>0.05);鱼藤酮(250 nmol/L)可使内源性PACAP mRNA的表达增高(P<0.05),神经肽PACAP单独作用对内源性PACAP mRNA的表达无影响(P>0.05),而与鱼藤酮和(或)PAC1受体拮抗剂PACAP6-27联合作用时均可使内源性PACAP mRNA的表达增高(P<0.05)。结论: 在鱼藤酮作用下,PC12细胞自身可以通过增高PAC1受体及内源性PACAP的表达部分代偿细胞的损伤。

       

      Abstract: Objective: To observe the selective alterations of endogenous pituitary adenylate cyclase-activating polypeptide(PACAP) and PAC1 receptor gene expression in PC12 cells induced by neurotoxin-Rotenone and neuropeptide-PACAP.Methods: Real-time polymerase chain reaction(RT-PCR) was performed to examine alterations of endogenous PACAP and PAC1 receptor gene expression in PC12 cells.Results: Our data demonstrated that rotenone significantly increased gene expression of endogenous PACAP and PAC1 receptor and only neuropeptide PACAP27 have no effects on the gene expressions of endogenous PACAP and PAC1 receptor;In base of rotenone-treatment,PACAP in presence of PAC1 antianognist,PACAP6-27 or without presence of it,promote increasing of PACAP expressions(P<0.01).Conclusions: Our study indicates that Our results indicated that Endogenous PACAP was shown to contribute to self-defense and compensatory mechanisms by regulating PACAP expression itself.

       

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