Abstract: Objective:To investigate the clinical value of fluorescent quatititive polymerase chain reaction(FQ-PCR) in the detection of hepatitis B Virus(HBV)-DNA.Methods:The positive rate and copies of HBV-DNA in 265 HBV patients and 150 healthy people were detected using FQ-PCR,which were compared with the serological markers of hepatitis B detected by enzyme linked immunosorbent assay.Results:The HBV-DNA was detected in all cases with different serological markers,the positive rate and copies of HBV-DNA in HBsAg,HBeAg and HBcAb(+) group and HBsAg and HBeAg(+) group were higher than those in other groups(PP>0.05),the copies of HBV in acute hepatitis patients were significantly higher than that in other groups(PConclusions:The HBV-DNA detected by FQ-PCR can directly reflect the HBV infection,judge the ability of replication and infection,guide the clinical medication and observe curative effects.