禹莉, 李玉云, 郝艳梅. 三氧化二砷对HL-60细胞MMP-2及MMP-9表达的影响[J]. 蚌埠医学院学报, 2011, 36(6): 547-550.
    引用本文: 禹莉, 李玉云, 郝艳梅. 三氧化二砷对HL-60细胞MMP-2及MMP-9表达的影响[J]. 蚌埠医学院学报, 2011, 36(6): 547-550.
    YU Li, LI Yu-yun, HAO Yan-mei. Effect of arsenic trioxide on expressions of matrix metalloproteinase 2 and 9 in HL-60 cells[J]. Journal of Bengbu Medical College, 2011, 36(6): 547-550.
    Citation: YU Li, LI Yu-yun, HAO Yan-mei. Effect of arsenic trioxide on expressions of matrix metalloproteinase 2 and 9 in HL-60 cells[J]. Journal of Bengbu Medical College, 2011, 36(6): 547-550.

    三氧化二砷对HL-60细胞MMP-2及MMP-9表达的影响

    Effect of arsenic trioxide on expressions of matrix metalloproteinase 2 and 9 in HL-60 cells

    • 摘要: 目的: 探讨三氧化二砷(arsenic trioxide,As2O3)对HL-60细胞基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)和MMP-9表达的影响。方法: 以HL-60细胞为对象,加入0.0、2.5、5.0、7.5 μmol/L As2O3干预48 h。RT-PCR检测MMP-2和MMP-9 mRNA的表达,Western blot检测MMP-2和MMP-9蛋白的表达。结果: HL-60细胞MMP-2、MMP-9 mRNA的表达随着As2O3浓度的增加而减少,5.0和7.5 μmol/L As2O3组与对照组差异均有统计学意义(P < 0.05~P < 0.01)。As2O3呈剂量依赖性抑制HL-60细胞MMP-2、MMP-9蛋白的表达,As2O3各组与对照组差异均有统计学意义(P < 0.05~P < 0.01)。结论: As2O3可抑制HL-60细胞MMP-2、MMP-9的表达,这一效应可能是As2O3治疗白血病的分子机制之一。

       

      Abstract: Objective: To investigate the effect of arsenic trioxide(As2O3) on expressions of matrix metalloproteinase 2 and 9 (MMP-2 and MMP-9) in HL-60 cells. Methods: HL-60 cells were treated with different concentrations of As2O3 for 48 hours. Semiquantitative reverse transcription polymerase chain reaction(RT-PCR) was used to examine the expressions of MMP-2 and MMP-9 mRNA. Western blot was used to evaluate the productions of MMP-2 and MMP-9 proteins. Results: The expressions of MMP-2 and MMP-9 mRNA in HL-60 cells were decreased after treated with As2O3. There were statistical significances between 5.0 and 7.5 μmol/L As2O3 groups and control group(P < 0.05-P < 0.01). The productions of MMP-2 and MMP-9 proteins were reduced dose-dependently when treated with As2O3. There was statistical significance between each experimental group and control group(P < 0.05-P < 0.01). Conclusions: As2O3 can inhibit the expressions of MMP-2 and MMP-9 in HL-60 cells. This effect might be one of the molecule mechanisms of As2O3 in the treatment of leukemia.

       

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