Abstract: Objective: To explore the resistance changes of Escherichia coli (E. coli) in urinary tract infection,and provide proofs about the reasonable use of drugs in clinic. Methods: Two hundred and thirty-four strains of E. coli in 2008 to 2010 annual urine specimen with extended-spectrum β-lactamase (ESBLs) detection and drug susceptibility test were detected,and the drug susceptibility results were analyzed. Results: The rate of enzyme production of E. coli showed a rising trend;the drug sensitivity test showed that the resistance existed in normal antibiotics was in different degrees except for Imipenem,and the rate of medical resisitance in ESBLs (+) bacteria was higher than in ESBLs (-). Conclusions: The resistance of urinary tract infection which caused by E. coli especially in ESBLs (+) strains is so serious. It should be strengthen in the detections of the medical resistance,which provide the references for the clinic rational drug use.