DNMT1和FOXO3在慢性牙周炎病人中表达水平及其对疾病严重程度的影响

喻祎; 胡艳荷; 江雅婷

DNMT1和FOXO3在慢性牙周炎病人中表达水平及其对疾病严重程度的影响

Expression levels of DNMT1 and FOXO3 in patients with chronic periodontitis and their influence on disease severity

摘要

摘要:
目的： 探究DNA甲基转移酶1（DNMT1）和叉头盒-O3（FOXO3）在慢性牙周炎（CP）病人中表达水平及其对疾病严重程度的影响。
方法： 纳入100例CP病人为研究对象（CP组）。根据美国疾病控制中心/美国牙周病学会（CDC/AAP）工作定义进一步将其分为轻度组（49例）和中重度组（51例）。另外纳入同期健康体检者100名为对照组。采用ELISA检测龈沟液DNMT1水平；采用荧光定量PCR测定龈沟液FOXO3的水平；采用Pearson法分析DNMT1、FOXO3水平与临床指标的关系；采用受试者工作特征（ROC）曲线评价DNMT1、FOXO3水平对CP病人严重程度的预测效能。
结果： CP组的DNMT1、FOXO3水平显著高于对照组（P ＜ 0.05）。中重度组牙龈指数、牙菌斑指数、沟出血指数、牙周袋深度、附件损耗、IL-6、IL-1β、TNF-α、CRP、DNMT1、FOXO3的水平高于轻度组（P ＜ 0.05）。CP病人龈沟液DNMT1与FOXO3、牙龈指数、沟出血指数、牙周袋深度、附件损耗、IL-6、IL-1β、TNF-α呈正相关（r = 0.537、0.609、0.632、0.516、0.524、0.628、0.650、0.558，P ＜ 0.05），与牙菌斑指数、CRP呈弱相关（r = 0.380、0.237，P ＜ 0.05）。龈沟液FOXO3与牙龈指数、沟出血指数、牙周袋深度、附件损耗、IL-6、IL-1β、TNF-α呈正相关（r = 0.602、0.598、0.504、0.511、0.619、0.638、0.537，P ＜ 0.05），与牙菌斑指数、CRP呈弱相关（r = 0.362、0.219，P ＜ 0.05）。DNMT1、FOXO3水平预测发生中重度CP的AUC分别为0.784、0.800，联合预测AUC（0.885）显著高于二者单独预测AUC（Z = 1.717、1.529，P ＜ 0.05）。
结论： CP病人龈沟液DNMT1、FOXO3水平较高，均与病人牙龈指数、沟出血指数、牙周袋深度、附件损耗、IL-6、IL-1β、TNF-α有关，能够反映CP的疾病活动度，有望成为中重度CP发生的预测因子。

Abstract:
Objective To investigate the expression levels of DNA methyltransferase 1 (DNMT1) and fork head box-O3FOXO3) in patients with chronic periodontitis (CP), and their influence on the severity of the disease.
Methods A total of 100 CP patients were selected as the CP group, and divided into the mild group (49 cases) and moderate and severe group (51 cases) according to the working definition of CDC/AAP. In addition, 100 cases of physical examination in the same period were selected as the control group. The DNMT1 level in gingival crevicular fluid was detected by ELISA, and the level of FOXO3 in gingival crevicular fluid was determined by fluorescence quantitative PCR. The Pearson method was used to analyze the relationship between DNMT1 and FOXO3 levels and clinical indicators. The predictive efficacy of DNMT1 and FOXO3 levels on the severity of CP patients was evaluated by receiver operating characteristic (ROC) curve.
Results The DNMT1 and FOXO3 levels in the CP group were higher than those in control group (P ＜ 0.05). Compared with the mild group, the gingival index, plaque index, sulcus bleeding index, periodontal pocket depth, accessory loss, and levels of IL-6, IL-1β, TNF-α, CRP, DNMT1 and FOXO3 in the moderate and severe group were obviously higher (P ＜ 0.05). The DNMT1 level in gingival crevicular fluid of CP patients was positively correlated with the FOXO3, gingival index, crevicular bleeding index, periodontal pocket depth, accessory loss, IL-6, IL-1β and TNF-α (r = 0.537, 0.609, 0.632, 0.516, 0.524, 0.628, 0.650, 0.558, P ＜ 0.05), and weakly correlated with the plaque index and CRP (r = 0.380, 0.237, P ＜ 0.05). The FOXO3 in gingival crevicular fluid was positively correlated with gingival index, sulcus bleeding index, periodontal pocket depth, accessory loss, IL-6, IL-1β and TNF-α (r = 0.602, 0.598, 0.504, 0.511, 0.619, 0.638, 0.537, P ＜ 0.05), and weakly correlated with the plaque index and CRP (r = 0.362, 0.219, P ＜ 0.05). The AUC of DNMT1 and FOXO3 levels in predicting the moderate and severe CP were 0.784 and 0.800, respectively, and the AUC of combined prediction (0.885) was significantly higher than that predicted by DNMT1 and FOXO3 alone (Z = 1.717, 1.529, P ＜ 0.05).
Conclusions The DNMT1 and FOXO3 levels in gingival crevicular fluid of patients with CP are high, which are related to the gingival index, crevicular bleeding index, periodontal pocket depth, accessory loss, IL-6, IL-1β and TNF-α, can reflect the disease activity of CP, and is expected to be a predictor of moderate and severe CP.

HTML全文

参考文献(18)

施引文献

资源附件(0)