去铁胺对小鼠糖尿病随意型皮瓣成活影响研究

Investigation of the influence of deferoxamine on the survival of a random pattern skin flap in diabetic mice

摘要: 目的：观察去铁胺（DFO）对小鼠糖尿病随意性皮瓣成活面积的影响。方法：C57/BL6小黑鼠被链脲佐菌素诱导成糖尿病后，在其背部制成1 cm3 cm随意性皮瓣，将DFO用PBS溶解后，腹腔注射，通过图像分析技术和激光多普勒分析皮瓣的成活率和血流状况。结果：DFO组皮瓣的存活面积（88.446.56）%明显大于PBS介质组（61.647.63）%（P＜0.01）。激光多普勒结果显示，DFO处理组皮瓣各部分血流值均大于PBS组（P＜0.01）。结论：DFO能够增加糖尿病C57/BL6小鼠背部随意皮瓣的血流量和存活面积。

Abstract: Objective:To investigate the inlluenc;e of deferoxamine(DFO) on the survival of random pattern skin flapin diabetic mice.Methods:Diabetes mellitus was induced with streptozotooin in C57/BL6 mice.A random pattern skin flap(1cmx3cm) was c;ranially designed and elevated on the back of C57 /BL6 mice.An image analysis technology was used to evaluate the survival rate of the skipflapand laser Doppler was used to survey the blood perfusion situation of skin flap.Results:Compared with control group(61.64士7.63)%,the survival area of the flapin DFO groupwas(88.44士6.56)%,which was significantly enlarged in seven days postoperatively (P0.01)Laser Doppler results displayed the blood flow in DFO groupwas higher than in control group(P0.01).Conclusions:DFO could improve flapmic;rooirc;ulation and increase its survival rate.