p16在人脑胶质瘤中的表达及其意义

Expression of p16 in human gliomas and its significance

摘要: 目的: 探讨p16在脑胶质瘤中的表达及其意义。方法: 采用免疫组织化学、核酸原位杂交和多重PCR的方法,检测68例脑胶质瘤和10例正常脑组织标本中p16的表达。结果: p16和mRNA在胶质瘤Ⅰ～Ⅱ级和Ⅲ～Ⅳ级的阳性表达,差异均有统计学意义(P<0.01～P<0.005),而多重PCR检测p16在胶质瘤Ⅰ～Ⅱ级和Ⅲ～Ⅳ级的阳性表达,差异无统计学意义(P>0.05)。结论: p16基因缺失可能与胶质瘤的发生有关;p16低表达在人脑胶质瘤的恶性进展中起重要作用,可作为预后评估指标之一。

Abstract: Objective: To explore the expression of p16 and its relationship with progression of human brain glioma.Methods: p16 protein was detected by immunohistochemistry in 68 cases of glioma and 10 cases of normal brain tissues.Nucleic acid hybridization in situ and multiplex polymerase chain reaction(M-PCR)were used to examine the expression of p16 mRNA and DNA in these tissues.Results: Expression of p16 protein and mRNA was higher in glioma grade Ⅰ-Ⅱ than in Ⅲ-Ⅳ(P<0.01-P<0.005).By using of M-PCR,the difference in expression of p16 between glioma grade Ⅰ-Ⅱ and Ⅲ-Ⅳ was not significant(P>0.05).Conclusions: Deletion of p16 perhaps had relationship with the occurrence of gliomas;the low expression of p16 may play an important role in progression in human brain gliomas.