Effect of Mycobacterium tuberculosis infection on CD14 expression in THP-1 cells
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摘要: 目的：探讨结核分枝杆菌(Mycobacterium tuberculosis，Mtb)感染THP-1细胞后对细胞表面白细胞分化抗原14(CD14)表达的影响。方法：Mtb以10∶1感染佛波醇酯(PMA)分化的THP-1细胞，建立Mtb感染THP-1细胞的模型。采用流式细胞术检测THP-1细胞、PMA分化的THP-1细胞及Mtb感染THP-1细胞CD14的表达。结果：THP-1细胞和PMA分化的THP-1细胞表面表达CD14的阳性率分别为5.5%和21.6%，对Mtb的吞噬率分别为7.7%和93.0%；Mtb感染的THP-1细胞表面表达CD14的阳性率为27.5%。结论：PMA分化后的THP-1细胞表面CD14的表达增加，Mtb感染后CD14的表达进一步增加，对Mtb的吞噬率增加。Abstract: Objective:To explore the changes of CD14 expression before and after THP-1 cells infected with Mycobacterium tuberculosis(Mtb). Methods:Mtb and phorbol myristate acetate(PMA)-differentiated THP-1 cells were incubated with the ratio of 10∶1,and Mtb infection cell model was established.The CD14 expression in THP-1 cells,PMA differentiated THP-1 cells and Mtb infected THP-1 cells were detected by flow cytometry. Results:The percentage of CD14 expression in THP-1 cells and PMA differentiated THP-1 cells was 5.5%,and 21.6%,respectively,whereas the percentage of Mtb phagocytosis rate was 7.7% and 93.0%,respectively.The percentage of CD14 expression in Mtb infected THP-1 cells was 27.5%. Conclusions:The CD14 expression in PMA differentiated THP-1 cells is increased,and the CD14 expression and Mtb phagocytosis rate in Mtb infected THP-1 cells are further increased.
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Keywords:
- Mycobacterium tuberculosis /
- THP-1 cells /
- CD14
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[1] World Health Organization.Global tuberculosis control-epidemiology,strategy,financing
[2] [2] Todd RT,Nadler,Schlossman SF.Antigens on human monocytes identified by monoclonal antibodies[J].J Immunol,1981,126(4):1435-1442.
[3] [3] Bufler P,Stiegler G,Schuchmann M,et al.Soluble lipopolysaccharide receptor(CD14) is released via two different mechanisms from human monocytes and CD14 transfectants[J].Eur J Immunol,1995,25(2):604-610.
[4] [4] Juan TS,Haian E,Kelley MJ,et al.Identification of a domain in soluble CD14 essential for lipopolysaccharide(LPS) signaling but not LPS binding[J].J Biol Chem,1995,270(29):17237-17242.
[5] [5] Zhang J,Jiang R,Takayama H,et al.Survival of virulent Mycobacterium tuberculosis involves preventing apoptosis induced by Bcl-2 upregulation and release resulting from necrosis in J774 macrophages[J].Microbiol Immunol,2005,49(9):45-52.
[6] [6] 金齐力,韦莉,姜丽娜,等.IL-15对人T细胞杀伤结核杆菌感染THP-1细胞的影响[J].中国人兽共患病学报,2010,26(10):926-930. [7] [7] Ezekowitz RA,Williams DJ,Koziel H,et al.Uptake of Pneumocystis carinii mediated by the macrophage mannose receptor[J].Nature,1991,351(6322):155-158.
[8] [8] Fontn P,Aris V,Ghanny S,et al.Global transcriptional profile of Mycobacterium tuberculosis during THP-1 human macrophage infection[J].Infect Immun,2008,76(2):717-725.
[9] [9] Juan TS,Haian E,Kelley MJ,et al.Identification of a domain in soluble CD14 essential for lipopolysaccharide(LPS) signaling but not LPS binding[J].J Biol Chem,1995,270(29):17237-17242.
[10] [10] Lynn WA,Liu Y,Golenbock DT.Neither CD14 nor serum is absolutely necessary for activation of mononuclear phagocytes by bacterial lipopolysaccharide[J].Infect Immun,1993,61(10):4452-4461.
[11] [11] Kurosaka K,Watanabe N,Kobayashi Y.Production of proinflammatory cytokines by resident tissue macrophages after phagocytosis of apoptotic cells[J].Cell Immunol,2001,211(1):1-7.
[12] [12] Gautier EL,Huby T,Saint-Charles F,et al.Enhanced dendritic cell survival altenuate lipopolysaccharide-induced immunosuppression and increases resistance to lethal endotoxic shock[J].J Immunol,2008,180(10):6941-6946.
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