朱晓骏, 孙学华, 刘顺庆, 高月求. 人自噬相关基因LC3B真核表达载体的构建及鉴定[J]. 蚌埠医学院学报, 2012, 36(2): 145-148.
    引用本文: 朱晓骏, 孙学华, 刘顺庆, 高月求. 人自噬相关基因LC3B真核表达载体的构建及鉴定[J]. 蚌埠医学院学报, 2012, 36(2): 145-148.
    ZHU Xiao-jun, SUN Xue-hua, LUN Shun-qing, GAO Yue-qiu. Construction and identification of eukaryotic expression vector recombined with human autopha}y-related LC3B gene[J]. Journal of Bengbu Medical College, 2012, 36(2): 145-148.
    Citation: ZHU Xiao-jun, SUN Xue-hua, LUN Shun-qing, GAO Yue-qiu. Construction and identification of eukaryotic expression vector recombined with human autopha}y-related LC3B gene[J]. Journal of Bengbu Medical College, 2012, 36(2): 145-148.

    人自噬相关基因LC3B真核表达载体的构建及鉴定

    Construction and identification of eukaryotic expression vector recombined with human autophay-related LC3B gene

    • 摘要: 目的:构建人自噬相关基因微管相关蛋白1轻链3B(microtubule-associated protein 1 light chain 3B,MAP1LC3B)真核表达栽体,并鉴定其生物学功能。方法:通过RT-PCR方法扩增得到人MAP1LC3B cDNA;应用基因重组技术构建pEGFP-N1-LC3B真核表达载体,通过酶切和测序进行鉴定;倒置荧光显微镜下观察EBBS诱导4 h后pEGFP-N1-LC3B表达载体转染的HepG2.2.15细胞质中GFP-LC3B分布的变化。结果:通过测序鉴定,pEGFP-N1-LC3B真核表达载体序列正确,编码框正确;转染后的HepG2.2.15细胞经EBBS诱导4 h后,倒置荧光显微镜检测发现GFP-LC3B由散在分布向点状分布改变。结论:成功构建了人自噬相关基因LC3B真核表达载体,为进一步研究自噬在乙型肝炎病毒中的作用机制奠定了基础。

       

      Abstract: Objective:To construet the eukaryotie expression vector with human autophagy-related mic;rotubule ssociated protein 1

       

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