Abstract: Objective:To observe the influence of genistein on spike encoding of cerebellar Purkenje cells after ischemia.Methods:The microelectrode and whole cell voltage clamp technique were used in the experiment.The changes of spike encoding of cerebellar Purkenje cells were detected during the ischemia after the cells were incubated with genistein at 200 mol/L for 10 min.Results:After ischemia,the values of inter-spike interval of sequential spikes were shortened and the values of standard deviation of spike timing were increased,the cells were overexcited,the accuracy of spike encoding was decreased,which were prevented by genistein.Conclusions:Genistein can prevent ischemia-induced over-excitation and stabilize the spike encoding of cerebellar Purkinje cells after ischemia.