Abstract: Objective:To explore the relationships of visfatin,nitric oxide(NO) and the metabolic markers and observe their roles in type 2 diabetes and the development in diabetic nephropathy(DN).Methods:Seventy-two patients with diabetes,in which 40 patients with diabetic nephropathy and 32 patients without complications were selected,and 24 healthy control cases whose age and body mass index(BMI) matched were also selected.The plasma level of visfatin was measured by enzyme-linked immunosorbent assay,and the level of plasma NO was measured by nitrate reductase method.While blood glucose,blood lipids,insulin,C peptide,proinsulin,glucagon,BMI,waist-hip ratio(WHR),high sensitivity C-reative protein and other related indicators were also measured.Insulin resistance was evaluated by insulin resistance index the homeostasis model assessment-IR(HOMA-IR),and insulin secretion index the HOMA- was used to evaluate -cell insulin secretion.The correlation between each index and microvascular complications of diabetes and diabetic nephropathy were analyzed.Results:The levels of plasma visfatin were 47.463.94,50.244.93,and 27.224.39 in diabetes cases without DN(nDN),diabetic cases with DN and control group respectively,and there had statistical differences among the three groups(P0.01);the plasma NO levels were 52.056.14,44.056.43,61.014.76 in diabetic patients nDN,diabetes with DN and control group,which had statistical differences(P0.01).The correlation analysis showed that there had positively correlation with the plasma visfatin level and the concentrations of NO,HOMA-IR,fasting insulin,waist circum ference,WHR,triglyceride in type 2 diabetes.Furtherly,HOMA-IR,WHR were independently associated with visfatin.Conclusions:Plasma visfatin and NO were related with type 2 diabetes and DN.