Abstract: Objective:To study the expressions and relationships of hypoxia-inducible factor-1(HIF-1) and B-cell-specific moloney leukemia virus insert site 1(Bmi-1)in diffuse large B cell lymphoma(DLBCL) tissue,and explore their roles and significance in the progression of carcinogenesis.Methods:The expressions of HIF-1 and Bmi-1 in 64 paraffin wax samples of DLBCL and 40 lymph node reactive hyperplasia tissue samples were detected by immunohistochemistry.The relationships and clinical features of HIF-1 and Bmi-1 in DLBCL were analyzed.Results:The postive expression rates of HIF-1 and Bmi-1 in DLBCL were 67.19% and 64.06% respectively,there were significantly statitical differences(P0.01)compared with the control.The significantly different expressions of HIF-1 and Bmi-1 were related with clinical stage and IPI(international prognostic index)(P0.01),and there were no obvious correlation with sex,age,out of lymph nodenode invasion and systemic symptoms of patient(P0.05).Conclusions:HIF-1 and Bmi-1 play a synergistic role in the progression of DLBCL,and can be used as an efficacious and prognostic reference indication of DLBCL.